mouse il 6 elisa kit  (Boster Bio)

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 indirectly suppressed osteoblast differentiation by enhancing the release of inflammatory factors from osteoclasts (A) Alizarin red staining and quantitative assessment of osteoblasts cultured for a duration of 21 days with supernatants derived from both control and Rv1509-treated osteoclasts. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05 [Student’s t test]). (B) Osteoblasts were subjected to treatment for 14 days with culture supernatants from control and Rv1509-treated osteoclasts, followed by ALP staining and subsequent quantification. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) The expression of osteoblast-specific mRNA was analyzed using RT-qPCR after a 7-day culture period with supernatants from control and Rv1509-treated osteoclasts. The data are shown as mean ± SEM. ( n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (D) ELISA was conducted to quantify the expression levels of inflammatory factors in osteoclasts from both the control and Rv1509-treated groups, while Sema4D expression was evaluated through RT-qPCR. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (E) Representative protein blots illustrating the expression of Runx2, Osterix, and Col1 in osteoblasts.

Article Snippet: Mouse IL-6 ELISA Kit , Boster , Cat#EK0483.

Techniques: Staining, Cell Culture, Derivative Assay, Control, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 Induces osteoclast differentiation and RANKL secretion by osteoblasts via TLR2 (A) After inducing osteoclast differentiation for four days in the presence of Rv1509 and C29, TRAP staining (scale bar: 400 μm) and F-actin staining (scale bar: 100 μm) were performed, followed by quantitative analysis. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (B) Following a four-day period of osteoclast differentiation stimulated by Rv1509 and C29, RT-qPCR was employed to evaluate the mRNA transcription levels of genes associated with osteoclasts. Additionally, ELISA was performed to measure the expression levels of inflammatory factors. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) After 14 days of osteoblast differentiation induced by Rv1509 and C29, ALP staining was performed, followed by Alizarin red staining after 21 days. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 3; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (D) The mRNA expression levels of osteoblast-related genes were analyzed using RT-qPCR after 14 days of differentiation in the presence of Rv1509 and C29. The data are shown as mean ± SEM. ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (E) Protein blots showing the representative expression levels of Ctsk, NFATc1, MMP9, and c-Fos in osteoclasts treated with Rv1509 and C29. (F) Protein blots showing representative levels of Col1, Osterix, and Runx2 in osteoblasts after treatment with Rv1509 and C29.

Article Snippet: Mouse IL-6 ELISA Kit , Boster , Cat#EK0483.

Techniques: Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet: Rv1509 induces osteolysis in mouse skulls (A) 3D reconstruction images of mouse skulls after two weeks of treatment with saline or Rv1509. The data are shown as mean ± SEM. (scale bar: 1 mm; n = 4; ∗, p < 0.05; ∗∗∗, p < 0.001 [Student’s t test]). (B) ELISA results of IL-6 and TNF-α levels in the mouse eye serum. The data are shown as mean ± SEM. ( n = 5; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (C) Representative images of H&E staining, Masson staining, TRAP staining, and immunohistochemical staining for p-p65 in mouse skull tissue(scale bar: 200 μm). (D) Quantitative analysis of TRAP staining, p-p65 immunohistochemical staining, and Masson staining. The data are shown as mean ± SEM. ( n = 3; ∗∗, p < 0.01; ∗∗∗, p < 0.001 [Student’s t test]). (E) Analysis of the calcein double-labeling experiment showing the effects of Rv1509 on bone formation and mineralization, scale bar: 50 μm. (F) Quantitative analysis of the calcein double-labeling experiment including bone mineralization deposit rate (MAR), mineralized surface area (MS/BS), and bone formation rate (BFR) ( n = 3). The data are shown as mean ± SEM. ( n = 3; ∗, p < 0.05; ∗∗∗, p < 0.001 [Student’s t test]).

Article Snippet: Mouse IL-6 ELISA Kit , Boster , Cat#EK0483.

Techniques: Saline, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemical staining, Labeling

Journal: iScience

Article Title: Mycobacterium tuberculosis specific protein Rv1509 modulates osteoblast and osteoclast differentiation via TLR2 signaling

doi: 10.1016/j.isci.2025.112107

Figure Lengend Snippet:

Article Snippet: Mouse IL-6 ELISA Kit , Boster , Cat#EK0483.

Techniques: Virus, Recombinant, Lysis, Staining, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, BIA-KA, RNA Sequencing, Software